This genomic DNA is purified from human placenta tissue of multiple donors and has an average size of around 500 bp up to 48 kb. Placenta DNA is rich of repetitive sequences and therefore especially suitable in hybridiziation methods, like Southern Blot Hybridization or FISH, to decrease non-specific hybridization of a probe or substrate. Due to the sequence variety, it can also be used as control material in Whole Genome Amplification, for the reduction of cross hybridization, construction of genomic libraries, background generation for PCR based sterilization assays and as blocking reagent for various applications.

With years of experience, BIORON has become one of the largest providers of Human DNA with customers all around the world, ranging from small research groups up to producing companies. The material has been tested for the absence of HIV, HBV, HCV and does not contain components of animal origin.

COT I Human DNA is made exclusively from placental DNA of male progeny. The production process is optimized so the highly repetitive elements of the Human DNA are enriched in an average size distribution of 50 - 300 bp. This gives BIORON´s COT I Human DNA unique characteristics that make it suitable for a wide range of applications.

It is used to suppress cross-hybridization to human repetitive sequences in various assays and enables the specific hybridizations to a chromosomal target site of probes. It is used in applications such as in-situ hybridizations (e.g. FISH) or filter / microarrays. In NGS technologies, COT I Human DNA is used as blocking reagent for non-specific sequences, which leads to higher on-target reading.

The product is available with different fragment size distributions, in various concentrations and in two analysis variants with specific measurement methods: photometric or  fluorometric. These parameters can be adjusted to customers´ needs in bulk quantities.

Each lot is tested for the absence of HIV, HBV, HCV and does not contain components of animal origin.

With BIORON polymerases you have got a wide range of enzymes for various applications in PCR, qPCR, Reverse Transcription and Isothermal Amplification. The enzymes show excellent processivity and stability, especially our Taq Polymerases. All polymerases are provided with advanced reaction buffers.

Our PCR Master Mixes are 2x concentrated (liquid), ready-to-use and optimized for various PCR, qPCR  and Reverse Transcription applications. The Ultimate Master Mixes are particularly suitable for Multiplex reactions and stable at +4 °C for several weeks. While primer, probes and template DNA are intended to be added by the customer, all other components (like HotStart Polymerase, PCR buffer, dNTPs and MgCl₂) are already included. All sets contain additional PCR grade water and MgCl₂.

Various solutions for your Reverse Transcription Setup:

• One-Step in one Tube: Easy, Fast, Sensitive – the Ultimate One-Step RT-PCR Master Mix combines reverse transcription and PCR amplification in one tube and one run!

• All inclusive: Everything needed to transcribe RNA into cDNA – the Ultimate cDNA Synthesis Kit with optional removal of genomic DNA (including Reverse Transcriptase, RNase Inhibitor, dNTPs, Random Hexamer and Oligo(dt) Primer, RNase Free Water and DNase Digest)

• You like it separately? No problem! With our single components you can assemble your personal setup for reverse transcription e. g. in research. From reverse transcriptase to random primers, please ask us for your quote.