Numaswitch is a biochemical production platform for easy and efficient peptide and protein production. Target proteins are fused to Switchtags which promote the high-titer production within E. coli cells as inclusion bodies. Switchtags trigger the efficient refolding of fusion proteins, while the Numacut TEV protease removes the desired targets tracelessly from the Switchtag. This technology resolves the challenge of low refolding efficiencies resulting in high product yields and purities.